Interaction of colloids with cells
Prof. Wolfgang Parak, Philipps-Universität Marburg
Some common sense statements about the interaction of colloidal nanoparticles with cells are given. While detail of this interaction strongly depends on the precise properties of the nanoparticles, the type of cells, conditions of incubation, etc. still some basic universal common sense dependences exist, which will be outlined. In general small, elongated, positively charged, soft particles are incorporated in vitro by cells to a higher extend than big, flat, negatively charged, stiff particles. After in vivo incorporation the environment of the nanoparticles drastically changes. In particular the nanoparticle may be exposed to different enzymes such as proteases. This may lead to changes in the surface chemistry of the nanoparticles. Consequences for active versus passive targeting will be discussed.
References about the presentation can be found in:
P. Rivera Gil, D. Jimenez de Aberasturi, V. Wulf, B. Pelaz, P. del Pino, Y. Zhao, J. de la Fuente, I. Ruiz de Larramendi, T. Rojo, X.-J. Liang, W. J. Parak, "The Challenge To Relate the Physicochemical Properties of Colloidal Nanoparticles to Their Cytotoxicity", Account of Chemical Research 46, 743–749 (2013).
R. Hartmann, M. Weidenbach, M. Neubauer, A. Fery, W. J. Parak, „Stiffness-Dependent In Vitro Uptake and Lysosomal Acidification of Colloidal Particles”, Angewandte Chemie International Edition 54, 1365 –1368 (2015)
B. Pelaz, P. del Pino, P. Maffre, R. Hartmann, M. Gallego, S. Rivera-Fernández, J. M. de la Fuente, G. U. Nienhaus, W. J. Parak, “Surface functionalization of nanoparticles with polyethylene glycol: effects on protein adsorption and cellular uptake”, ACS Nano 9, 6996–7008 (2015).
M. Chanana, P. Rivera Gil, M. A. Correa-Duarte, L. M. Liz-Marzán. W. J. Parak, "Physicochemical Properties of Protein-Coated Gold Nanoparticles in Biological Fluids and Cells before and after Proteolytic Digestion", Angewandte Chemie International Edition 52, 4179–4183 (2013).
W. G. Kreyling, A. M. Abdelmonem, Z. Ali, F. Alves, M. Geiser, N. Haberl, R. Hartmann, S. Hirn, K. Kantner, D. Jimenez de Aberasturi, G. Khadem-Saba, J.-M. Montenegro, J. Rejman, T. Rojo, I. Ruiz de Larramendi, R. Ufartes, A. Wenk, W. J. Parak, "In vivo integrity of polymer-coated gold nanoparticles", Nature Nanotechnology 10, 619–623 (2015).